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Journal of Clinical Endocrinology & Metabolism, Vol 56, 992-997, Copyright © 1983 by Endocrine Society

ARTICLES

Human plasma beta-endorphin-like peptides: a rapid, high recovery extraction technique and validation of radioimmunoassay

CA Cahill, JD Matthews and H Akil

This is a report of the development, calibration, and validation of a series of techniques required to measure beta-endorphin (beta-END)-like immunoreactivity in human plasma, including sieve and affinity chromatography. The RIA, which uses the antibody Brenda, is very sensitive (IC50 = 5-15 fmol/tube at a final concentration of 1:40,000). The extraction process, which uses the Sep-Pak C18 cartridge (Waters Associates, Inc.), is simple and rapid and has a recovery rate of more than 90%. It extracts proopiomelanocortin, beta-lipotropin, and beta- END. Physiological validation was provided by the measurement of beta- END-like immunoreactivity in a pool of plasma of normal humans (2.25 fmol/ml plasma), two pregnant women at term (9.5 and 10.75 fmol/ml), and one patient with Nelson's disease (2 pmol/ml plasma).


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