| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Journal of Clinical Endocrinology & Metabolism, Vol 56, 962-968, Copyright © 1983 by Endocrine Society
ARTICLES |
E Markoff, P Zeitler, S Peleg and S Handwerger
An enriched fraction of human decidual cells that synthesizes and releases human PRL (hPRL) was obtained by isopycnic centrifugation of collagenase- and hyaluronidase-dispersed cells through Percoll. The cells that synthesized and released hPRL banded at a density of 1.017- 1.045 g/ml, an area of the gradient comprising only a small percentage of the total decidual DNA. The enriched cells formed distinct colonies in culture and contained hPRL, as evidenced by indirect immunofluorescent staining with anti-PRL serum. Plated at a density of 5.0 x 10(5) cells/well, the cells produced hPRL at a mean rate of 8.1 +/- 1.1 ng/microgram DNA . 24 h (mean +/- SD) for 8 days. Like decidual explants, the enriched cells responded to phospholipase A2 (0.1 U/ml) with a 54% decrease in hPRL release and to placental conditioned medium (0.5 mg protein/ml medium) with a 62% increase. Insulin (8.3 x 10(-7) M), progesterone (10(-5) - 10(-12) M), and estradiol (10(-5) - 10(-12) M) did not affect hPRL release over 6 days. These results indicate that enriched PRL-releasing cells, obtained by the isopycnic centrifugation of collagenase- and hyaluronidase-dispersed cells, are a useful model for the study of the synthesis and release of PRL.
This article has been cited by other articles:
![]() |
I. Ihnatovych, W. Hu, J. L. Martin, A. T. Fazleabas, P. de Lanerolle, and Z. Strakova Increased Phosphorylation of Myosin Light Chain Prevents in Vitro Decidualization Endocrinology, July 1, 2007; 148(7): 3176 - 3184. [Abstract] [Full Text] [PDF] |
||||
![]() |
P. Cameo, M. Szmidt, Z. Strakova, P. Mavrogianis, K. L. Sharpe-Timms, and A. T. Fazleabas Decidualization Regulates the Expression of the Endometrial Chorionic Gonadotropin Receptor in the Primate Biol Reprod, November 1, 2006; 75(5): 681 - 689. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. J. Kim, H. S. Taylor, G. E. Akbas, I. Foucher, A. Trembleau, R. C. Jaffe, A. T. Fazleabas, and T. G. Unterman Regulation of Insulin-Like Growth Factor Binding Protein-1 Promoter Activity by FKHR and HOXA10 in Primate Endometrial Cells Biol Reprod, January 1, 2003; 68(1): 24 - 30. [Abstract] [Full Text] [PDF] |
||||
![]() |
Z. Strakova, S. Srisuparp, and A. T. Fazleabas Interleukin-1{beta} Induces the Expression of Insulin-Like Growth Factor Binding Protein-1 during Decidualization in the Primate Endocrinology, December 1, 2000; 141(12): 4664 - 4670. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y. Kanda, H. Jikihara, E. Markoff, and S. Handwerger Interleukin-2 Inhibits the Synthesis and Release of Prolactin from Human Decidual Cells J. Clin. Endocrinol. Metab., February 1, 1999; 84(2): 677 - 681. [Abstract] [Full Text] |
||||
![]() |
R. L. Jones, H. O. D. Critchley, J. Brooks, H. N. Jabbour, and A. S. McNeilly Localization and Temporal Expression of Prolactin Receptor in Human Endometrium J. Clin. Endocrinol. Metab., January 1, 1998; 83(1): 258 - 262. [Abstract] [Full Text] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |