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Characterization of the Synthesis and Release of Prolactin by an Enriched Fraction of Human Decidual Cells^*

Departments of Pediatrics and Physiology, Duke University Medical Center, Durham, North Carolina, 27710; and the Department of Hormone Research, The Weizmann Institute of Science (S.P.) Rehovot, Israel

Address all correspondence and requests for reprints to: Stuart Handwerger, M.D., Duke University Medical Center, Department of Pediatrics, Box 3080, Durham, North Carolina 27710.

An enriched fraction of human decidual cells that synthesizes and releases human PRL (hPRL) was obtained by isopycnic centrifugation of collagenase- and hyaluronidasedispersed cells through Percoll. The cells that synthesized and released hPRL banded at a density of 1.017–1.045 g/ml, an area of the gradient comprising only a small percentage of the total decidual DNA. The enriched cells formed distinct colonies in culture and contained hPRL, as evidenced by indirect immunofluorescent staining with anti-PRL serum. Plated at a density of 5.0 x 10⁵ cells/well, the cells produced hPRL at a mean rate of 8.1 ± 1.1 ng/µg DNA·24 h (mean ± SD) for 8 days. Like decidual explants, the enriched cells responded to phospholipase A₂ (0.1 U/ml) with a 54% decrease in hPRL release and to placental conditioned medium (0.5 mg protein/ml medium) with a 62% increase. Insulin (8,3 x 10^–7M), progesterone (10^–5–10^–12 M), and estradiol (10^–5–10^–12 M) did not affect hPRL release over 6 days. These results indicate that enriched PRL-releasing cells, obtained by the isopycnic centrifugation of collagenase- and hyaluronidase-dispersed cells, are a useful model for the study of the synthesis and release of PRL. (J Clin Endocrinol Metab 56: 962, 1983)

^* This work was supported by NIH Research Grants HD-06153, HD-15201, and GM-07171.

Received September 20, 1982.

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