help button home button Endocrine Society JCEM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

Journal of Clinical Endocrinology & Metabolism Vol. 56, No. 5 962-968
doi:10.1210/jcem-56-5-962
Copyright © 1983 by the Endocrine Society.
This Article
Right arrow Full Text (PDF)
Right arrow Submit a related Letter to the Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Markoff, E.
Right arrow Articles by Peleg, S. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Markoff, E.
Right arrow Articles by Peleg, S. H.

Characterization of the Synthesis and Release of Prolactin by an Enriched Fraction of Human Decidual Cells*

Edith Markoff, Philip Zeitler, Sarah Peleg and Stuart Handwerger Peleg

Departments of Pediatrics and Physiology, Duke University Medical Center, Durham, North Carolina, 27710; and the Department of Hormone Research, The Weizmann Institute of Science (S.P.) Rehovot, Israel

Address all correspondence and requests for reprints to: Stuart Handwerger, M.D., Duke University Medical Center, Department of Pediatrics, Box 3080, Durham, North Carolina 27710.

An enriched fraction of human decidual cells that synthesizes and releases human PRL (hPRL) was obtained by isopycnic centrifugation of collagenase- and hyaluronidasedispersed cells through Percoll. The cells that synthesized and released hPRL banded at a density of 1.017–1.045 g/ml, an area of the gradient comprising only a small percentage of the total decidual DNA. The enriched cells formed distinct colonies in culture and contained hPRL, as evidenced by indirect immunofluorescent staining with anti-PRL serum. Plated at a density of 5.0 x 105 cells/well, the cells produced hPRL at a mean rate of 8.1 ± 1.1 ng/µg DNA·24 h (mean ± SD) for 8 days. Like decidual explants, the enriched cells responded to phospholipase A2 (0.1 U/ml) with a 54% decrease in hPRL release and to placental conditioned medium (0.5 mg protein/ml medium) with a 62% increase. Insulin (8,3 x 10–7M), progesterone (10–5–10–12 M), and estradiol (10–5–10–12 M) did not affect hPRL release over 6 days. These results indicate that enriched PRL-releasing cells, obtained by the isopycnic centrifugation of collagenase- and hyaluronidase-dispersed cells, are a useful model for the study of the synthesis and release of PRL. (J Clin Endocrinol Metab 56: 962, 1983)

* This work was supported by NIH Research Grants HD-06153, HD-15201, and GM-07171.

Received September 20, 1982.




This article has been cited by other articles:


Home page
Biol. Reprod.Home page
I. Ihnatovych, M. Livak, J. Reed, P. de Lanerolle, and Z. Strakova
Manipulating Actin Dynamics Affects Human In Vitro Decidualization
Biol Reprod, July 1, 2009; 81(1): 222 - 230.
[Abstract] [Full Text] [PDF]


Home page
EndocrinologyHome page
I. Ihnatovych, W. Hu, J. L. Martin, A. T. Fazleabas, P. de Lanerolle, and Z. Strakova
Increased Phosphorylation of Myosin Light Chain Prevents in Vitro Decidualization
Endocrinology, July 1, 2007; 148(7): 3176 - 3184.
[Abstract] [Full Text] [PDF]


Home page
Biol. Reprod.Home page
P. Cameo, M. Szmidt, Z. Strakova, P. Mavrogianis, K. L. Sharpe-Timms, and A. T. Fazleabas
Decidualization Regulates the Expression of the Endometrial Chorionic Gonadotropin Receptor in the Primate
Biol Reprod, November 1, 2006; 75(5): 681 - 689.
[Abstract] [Full Text] [PDF]


Home page
Biol. Reprod.Home page
J. J. Kim, H. S. Taylor, G. E. Akbas, I. Foucher, A. Trembleau, R. C. Jaffe, A. T. Fazleabas, and T. G. Unterman
Regulation of Insulin-Like Growth Factor Binding Protein-1 Promoter Activity by FKHR and HOXA10 in Primate Endometrial Cells
Biol Reprod, January 1, 2003; 68(1): 24 - 30.
[Abstract] [Full Text] [PDF]


Home page
EndocrinologyHome page
Z. Strakova, S. Srisuparp, and A. T. Fazleabas
Interleukin-1{beta} Induces the Expression of Insulin-Like Growth Factor Binding Protein-1 during Decidualization in the Primate
Endocrinology, December 1, 2000; 141(12): 4664 - 4670.
[Abstract] [Full Text] [PDF]


Home page
J. Clin. Endocrinol. Metab.Home page
Y. Kanda, H. Jikihara, E. Markoff, and S. Handwerger
Interleukin-2 Inhibits the Synthesis and Release of Prolactin from Human Decidual Cells
J. Clin. Endocrinol. Metab., February 1, 1999; 84(2): 677 - 681.
[Abstract] [Full Text]


Home page
J. Clin. Endocrinol. Metab.Home page
R. L. Jones, H. O. D. Critchley, J. Brooks, H. N. Jabbour, and A. S. McNeilly
Localization and Temporal Expression of Prolactin Receptor in Human Endometrium
J. Clin. Endocrinol. Metab., January 1, 1998; 83(1): 258 - 262.
[Abstract] [Full Text]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1983 by The Endocrine Society