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Radioimmunoassay and Bioassay of Human Growth Hormone and Human Prolactin^*

Department of Pediatrics and Department of Endocrinology, Toranomon Hospital Tokyo, Japan 107;
Department of Biochemistry, University of British Columbia Vancouver, British Columbia, Canada V6T1W5;
Department of Cancer Endocrinology, Cancer Control Agency of British Columbia Vancouver, British Columbia, Canada V5Z3J3;
Department of Physiology (H.G.F.), Faculty of Medicine, University of Manitoba Winnipeg, Manitoba, Canada R3E 0W3

The rat lymphoma cell line was used to bioassay human GH (hGH) in 30 serum samples from 4 acromegalic patients and human PRL (hPRL) in 25 sera from 5 hyperprolactinemic patients. The ratio of the bioassay and RIA estimates of hGH was 0.96 ± 0.13 (mean ± SD) and of hPRL was 1.06 ± 0.14. There was no significant difference in the ratio of the bioassay and RIA estimates of hGH or hPRL during the time course of TRH tests in acromegalic or in hyperprolactinemic patients, respectively.

^* This research was supported by grants from the MRC of Canada and USPHS Grant HD-07843-09.

Received April 20, 1982.