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Journal of Clinical Endocrinology & Metabolism, Vol 55, 44-48, Copyright © 1982 by Endocrine Society

ARTICLES

Monoclonal antibody to human thyrotropin

EC Ridgway, LJ Ardisson, MJ Meskell and M Mudgett-Hunter

Using the technique of somatic cell fusion, a monoclonal antibody to human TSH (hTSH) has been produced. The monoclonal antibody (anti-hTSH 1/1) has an affinity (Kd = 4.04 x 10(-9) M/liter) for hTSH which is slightly less than that of the polyclonal antisera (Kd = 9.8 x 10(-10) M/liter) derived from the same mouse used for the fusion experiment. The beta-subunit of hTSH displays nearly complete cross-reaction, but has a lower affinity (Kd = 2.6 x 10(-8) M/liter) for the monoclonal antibody. hCG and its alpha- and beta-subunits as well as human LH do not cross-react with this antibody. The high specificity of this antibody will make it useful as an immunofluorescent probe of hTSH- secreting cells as well as in the immunoabsorption and purification of hTSH.


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R. Zhang and L. J. DeGroot
A Monoclonal Antibody against Rat Calcitonin Inhibits the Growth of a Rat Medullary Thyroid Carcinoma Cell Line in Vitro
Endocrinology, April 1, 1997; 138(4): 1697 - 1703.
[Abstract] [Full Text] [PDF]


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