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Journal of Clinical Endocrinology & Metabolism, Vol 52, 586-588, Copyright © 1981 by Endocrine Society

ARTICLES

beta-Endorphin 61-91 and other beta-endorphin-immunoreactive peptides in human semen

B Sharp and AE Pekary

Human semen contains large amounts of authentic beta-endorphin and other beta-endorphin immunoreactive peptides. To eliminate the non- specific effects of semen in the beta-endorphin RIA, the beta-endorphin immunoreactive peptides were extracted with octadecylsilane-C18 reverse phase beads. beta-Endorphin immunoreactivity was parallel to the RIA standard curve. Sephadex G-50 gel chromatography showed that 93% of the immunoreactive material coeluted with beta-endorphin. Reversed phase high pressure liquid chromatography of semen extract demonstrated several peaks of beta-endorphin immunoreactivity; one of these was coincident with synthetic h-beta-endorphin61-91.


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J. Pintar, B. Schachter, A. Herman, S Durgerian, and D. Krieger
Characterization and localization of proopiomelanocortin messenger RNA in the adult rat testis
Science, August 10, 1984; 225(4662): 632 - 634.
[Abstract] [PDF]


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