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Journal of Clinical Endocrinology & Metabolism, Vol 52, 367-369, Copyright © 1981 by Endocrine Society

ARTICLES

Radioimmunoassay of leucine-enkephalin-like substance in human and canine plasma

SW Ryder and J Eng

Fasting human plasma immunoreactive leu-enkephalin (ir-leu-enkephalin) measured by radioimmunoassay averages 54+/-10 pg/ml. The method depends on acidification of plasma to protect against destruction of the peptide, adsorption to XAD-2 resin, extraction from the resin by aqueous methanol and concentration by evaporation. Plasma enkephalin in the dog increased from 13 to 56 pg/ml following insulin-induced hypoglycemia.




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Endocrinology Endocrine Reviews J. Clin. End. & Metab.
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Copyright © 1981 by The Endocrine Society