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Effects of Adenosine on Lipolysis in Human Subcutaneous Fat Cells^*

Department of Internal Medicine, Maria Hospital, and Department of Medical Chemistry, University of Helsinki Helsinki, Finland

Address all correspondence and requests for reprints to: Jorma J. Ohisalo, M.D., Department of Medical Chemistry, Siltavuorenpenger 10 A, SF-00170 Helsinki 17, Finland.

Adenosine inhibited isoproterenol-stimulated lipolysis in isolated human adipocytes. At a 1-µM stimulator concentration, half-maximal inhibition was observed at about 0.3 µM adenosine. The addition of adenosine deaminase to the incubation medium enhanced the stimulation of lipolysis by 1 µM isoproterenol; the enzyme was also lipolytic by itself. The combined effect of adenosine deaminase and isoproterenol was strongly inhibited by 250 nM N⁶-(phenylisopropyl) adenosine; the latter compound cannot be deaminated by adenosine deaminase. This inhibition was almost totally overcome by increasing the concentration of the catecholamine to 10 µM. These results suggest that human fat cells excrete adenosine, and this nucleoside reversibly inhibits the stimulation of lipolysis by catecholamines.

^* This work was supported by The Nutrition Research Foundation of Finnish Sugar Co., Ltd.

Received July 21, 1980.

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