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A Rapid, Centrifugation-Free Radioimmunoassay Specific for Human Chorionic Gonadotropin Using Glass Beads as Solid Phase^*

KLAUS G. POST, ASIT K. ROY, LARS L. CEDERQVIST and BRIJ B. SAXENA

Department of Medicine, Division of Endocrinology, and the Department of Obstetrics and Gynecology, Cornell University Medical College New York, New York 10021

Address requests for reprints to: Brij B. Saxena, Cornell University Medical College, 1300 York Avenue, New York, New York 10021.

A simple, specific, rapid, centrifugation-free solid phase RIA for hCG has been developed. Purified -globulin isolated from antisera produced against hCGβ in rabbits is covalently linked to glutaraldehyde-activated aminopropyl glass beads. The entire assay can be completed within 3 h at room temperature. The sensitivity (1 ng/ml), precision (intra- and interassay variabilities, <10% and <15%, respectively), and specificity (0.4% cross-reactivity with LH) are comparable to other methods. The hCG levels determined by the present method show good correlation with those obtained by another RIA and by radioreceptorassay. The present method also provides the basis for the development of similar rapid and sensitive assays of other hormones.

^* Presented in part at the 63rd FASEB Meeting, Dallas, TX, April 1979.

Supported by Fellowship PO 200/1 from the Deutsche Forschungs gemeinschaft, Bonn Bad-Godesberg, Germany. Present address: Department of Obstetrics and Gynecology, Division of Gynaecological Endocrinology, Sterility, and Family Planning, Klinikum Steglitz, Free University of Berlin, Germany.

Received May 14, 1979.