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Journal of Clinical Endocrinology & Metabolism Vol. 48, No. 2 271-278
doi:10.1210/jcem-48-2-271
Copyright © 1979 by the Endocrine Society.
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Immunoreactive Somatomedin A in Human Serum*

KERSTIN HALL, JOHNNY BRANDT, GöSTA ENBERG and LINDA FRYKLUND

Department of Endocrinology, Karolinska Hospital (K.H., G.E.), and the Polypeptide Laboratory, AB Kabi (J.B., L.F.) Stockholm, Sweden

Address requests for reprints to: Kerstin Hall, M.D., Department of Endocrinology, Karolinska Hospital, S-104 01 Stockholm, Sweden.

A RIA has been developed for somatomedin A (SM-A) utilizing Sepharose-bound antibodies. This assay, measuring SM-A, the insulin-like growth factors 1 and 2, and somatomedin C, allows determination in serum samples. In comparison with a serum standard, the mean serum levels in patients with acromegaly or GH deficiency and healthy subjects were 8.7 ± 0.7 (n = 25), 0.24 ± 0.02 (n = 25), and 1.15 ± 0.11 U/ml, respectively. The correlation coefficient between immunoreactive SM-A and SM-A by radioreceptor assay was highly significant (r = 0.93), although the potency ratio of SM-A between the two groups of patients was higher in the RIA than in the radioreceptor assay. Gel chromatography revealed that SM-A in acromegalic serum is bound to a carrier protein which is absent in patients with GH deficiency. After gel chromatography at low pH, 90% of applied immunoreactive SM-A was recovered in the low molecular weight fraction and consisted mainly of neutral polypeptides. (J Clin Endocrinol Metab 48: 271, 1979)

* This work was supported by grants from Swedish Medical Research Council (19X-4224) and Svenska Läkarsällskapet.

Received February 27, 1978.






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Copyright © 1979 by The Endocrine Society