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Journal of Clinical Endocrinology & Metabolism, Vol 46, 891-896, Copyright © 1978 by Endocrine Society

ARTICLES

A bioassay capable of measuring 1 picogram of 1,25-dihydroxyvitamin D3

PH Stern, AJ Hamstra, HF DeLuca and NH Bell

Fetal rat bones in organ culture constitute a sensitive system for assay of the vitamin D metabolite, 1 alpha,25-dihydroxyvitamin D3. Significant bone resorption is obtained with as little as 2 pg 1,25- dihydroxyvitamin D3 after 48 h of culture and with 1 pg after 64 h of culture. In the current study, organ cultures of fetal rat bone are used as a bioassay for 1,25-dihydroxyvitamin D i normal human plasma, which was prepared for assay by extraction with dichloromethane, chromatography on Sephadex LH-20, and purification on silicic acid by high pressure liquid chromatography. The concentration of 1 alpha,25- dihydroxyvitamin D3 in normal adult human plasma was 24.8 +/- 2.0 pg/ml (n = 19) by this assay.




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