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Journal of Clinical Endocrinology & Metabolism, Vol 46, 105-113, Copyright © 1978 by Endocrine Society

ARTICLES

The development and application of a direct radioimmunoassay for plasma aldosterone using 125I-labeled ligand--comparison of three methods

EA Al-Dujaili and CR Edwards

A direct, rapid, sensitive, and highly specific radioimmunoassay for plasma aldosterone has been developed using aldosterone conjugated to [125I]histamine as ligand and a highly specific aldosterone antiserum. This assay permits the direct measurement of plasma aldosterone without preliminary extraction or purification steps and hence allows a single technician to assay 500 samples in a week. An excellent correlation was obtained between the results of the direct assay and the levels measured after paper chromatography (n = 43, r = 0.99, P less than 0.001) or after extraction (n = 43, r = 0.99, P less than 0.001). The coefficients of variation for intra-assay and inter-assay determinations of samples from a normal plasma pool were 7.6% and 9.3%, respectively.


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