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Journal of Clinical Endocrinology & Metabolism, Vol 45, 1128-1130, Copyright © 1977 by Endocrine Society

ARTICLES

The use of tetrahydrocortisone-3-beta-D-glucuronide in the measurement of urinary 17-hydroxycorticosteroids

HE Hadd

THE 3-G was added to H2O and 56 urines, and the recovered THE was measured by the Porter-Silber method. The recovery from H2O was quantitative (98 +/- 2%), but highly variables from urine, ranging from 35 to 100%. The necessity of the proper standard in analysis of urinary steroid glucuronides was demonstrated. The presence in urine of endogenous inhibitors to beta-glucuronidase was confirmed.




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Endocrinology Endocrine Reviews J. Clin. End. & Metab.
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Copyright © 1977 by The Endocrine Society