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Journal of Clinical Endocrinology & Metabolism Vol. 44, No. 5 952-962
doi:10.1210/jcem-44-5-952
Copyright © 1977 by the Endocrine Society.
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Heterogeneity of Circulating C-Peptide

HIDESHI KUZUYA, PETRA M. BLIX, DAVID L. HORWITZ, ARTHUR H. RUBENSTEIN, DONALD F. STEINER, CHRISTIAN BINDER and OLE K. FABER

Departments of Medicine and Biochemistry, University of Chicago, Chicago, Illinois, and Hvidtfre Hospital, Emiliekildevej 1 DK-2930 Klampenborg, Denmark

Reprint requests to: Arthur H. Rubenstein, M.D., Department of Medicine, University of Chicago, 950 East 59th Street, Chicago, Illinois 60637.

Serum C-peptide levels vary when measured with different immunoassay systems. In order to assess the factors contributing to this finding, serum C-peptide was measured in two assays utilizing different antisera, but the same standards and labeled peptide. The antisera were characterized with synthetic C-peptide fragments and their reactivities towards some of these fragments differed. The results of dilution and recovery tests and stability of the C-peptide during storage showed differences between the two assays. Gel filtration experiments indicated heterogeneity within the major C-peptide peak, and, in addition, a smaller peak of lower molecular weight material was present in some sera. Although degradation of serum C-peptide may occur during storage or with freezing and thawing, fragments of C-peptide may also be secreted or arise during in vivo metabolism.

Supported by grants from the Bertha and Henry Brownstein Foundation, the Juvenile Diabetes Foundation, USPHS (AM 13941 and AM 19206) and the Diabetes Endocrinology Center (AM 17046) at the University of Chicago. D. L. Horwitz is a recipient of a Research Career Development Award and A. H. Rubenstein is an Established Investigator of the American Diabetes Association.

Received October 15, 1976.






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Copyright © 1977 by The Endocrine Society