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The Concentration of Angiotensins I and II in Blood from the Pulmonary Artery and Left Ventricle of Man

Medical Research Council Blood Pressure Unit, Western Infirmary, Glasgow Gil 6NT Scotland

The concentrations of angiotensin I (AI) and II (All) were determined by radioimmunoassay in blood from the main pulmonary artery (MPA) and left ventricle (LV) often subjects with rheumatic valvular heart disease. The levels of AI were consistently higher in MPA plasma (21.8±2.4 pmol/1) than in LV plasma (14.7±2.0 pmol/1), paired t, P<0.001. The levels of All were consistently lower in MPA plasma (21.8±4.7 pmol/1) than in LV plasma (33.8±7.2 pmol/1) paired t, P<0.001. The All antiserum cross-reacted with three metabolites of the hormone, [des-Asp¹]angiotensin II, [des-(Asp¹ Arg²)]angiotensin II [des-(Asp²Arg²Val³)]angiotensin II. To characterize the nature of circulating All immunoreactive material, paper chromatography was used to separate All from its immunoreactive metabolites. The results showed that 84-100%of the All immunoreactive material from both MPA and LV plasma chromatographed with the mobility of authentic angiotensin II. The mean pulmonary conversion of endogenous AI was 33±4.8%and the net extraction of All by peripheral tissues was 33±4.1%.

Reprint requests to: Dr. Peter Semple, MRC Blood Pressure Unit, Western Infirmary, Glasgow, Gil 6NT, Scotland.

Received October 14, 1976.