This Article Full Text (PDF) Submit a related Letter to the Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by RECHLER, M. M. Articles by FRYKLUND, L. Search for Related Content PubMed PubMed Citation Articles by RECHLER, M. M. Articles by FRYKLUND, L.

Identification of a Receptor for Somatomedin-Like Polypeptides in Human Fibroblasts^*

MATTHEW M. RECHLER, S. PETER NISSLEY, JUDITH M. PODSKALNY, ALAN C. MOSES and LINDA FRYKLUND

Diabetes Branch, National Institute of Arthritis, Metabolism, and Digestive Diseases, National Institutes of Health, Bethesda Maryland 20014
Metabolism Branch, National Cancer Institute, National Institutes of Health, Bethesda Maryland 20014
AB KABI, The Becip Polypeptide Laboratory, Stockholm Sweden

We have demonstrated a specific receptor for somatomedin-like growth polypeptides in human fibroblasts in culture using the closely related polypeptide, multiplication stimulating activity (MSA), as the radioligand. Polypeptides purified from human plasma, somatomedin A and acid soluble nonsuppressible insulin-like activity (NSILA-s), competed potently for ¹²⁵I-MSA binding, as did unlabeled MSA. Although insulin and proinsulin also strongly inhibited MSA binding, the propertiesof the growth peptide receptor differed from those of the human fibroblast insulin receptor. Somatomedin A, NSILA-s, MSA, insulin and proinsulin all stimulated the incorporation of [³H]thymidine into DNA in human fibroblasts. We propose that these polypeptides induce DNA synthesis through their interaction with the growth peptide receptor.

^* preliminary account of these results has been presented at the Thirty-third Annual Meeting of the American Federation for Clinical Research, Atlantic- City, New Jersey, May 1, 1976

Received November 17, 1975.

This article has been cited by other articles:

				K.-C. Leung, G. Johannsson, G. M. Leong, and K. K. Y. Ho Estrogen Regulation of Growth Hormone Action Endocr. Rev., October 1, 2004; 25(5): 693 - 721. [Abstract] [Full Text] [PDF]