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Immunoreactivity and Serum Destruction of N^3imMethyl-TRH

Endocrine Section, Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia Philadelphia, Pennsylvania 19174

Pyroglu-N^31mmethyl-histidyl-prolineamide (MeTRH) is a more potent stimulator of thyrotropin and prolactin secretion than thyrotropinreleasing hormone (TRH). In this study, the immunoreactivity of MeTRH and its susceptibility to destruction by serum were investigated. When tested in radioimmunoassays employing four different anti- TRH sera, MeTRH had 25 to 60% of the immunoreactivity of TRH. Serum destroyed the immunoreactivity of MeTRH at one-half of the rate at which TRH was destroyed. Slow degradation alone would not appear to explain the potency of MeTRH.

Supported by USPHS grant AM 14039.

Received September 27, 1976.