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Studies of the Composition and Radioreceptor Activity of "Big" and "Little" Human Growth Hormone

Division of Endocrinology and Metabolism, Department of Medicine, Albany Medical College Albany, New York 12208

Immunoreactive grRowth hormone (GH) from human pituitary and plasma contains "big" (BGH) and "little" (LGH) components. BGH itself consists of a "urea-labile" form and a "urea-stable" form (usBGH). In the present study we determined the amino acid composition of a BGH preparation containing both the urea-labile and urea-stable components and found it to be indistinguishable from that of LGH. This finding, coupled with observations of others, suggests that urea-labile BGH is a simple LGH dimer and that usBGH is a disulfide dimer.

We have prepared LGH, BGH, and usBGH from human pituitary GH, and studied their radioreceptor activity, in relation to their immunoreactivity, in plasma membrane systems from rabit, rat and human liver and rabbit mammary gland. When ¹²⁵I-LGH was used as the radioligand, LGH and usBGH caused parallel displacement. usBGH was 60–74% as active as LGH in the animal preparations, while in human liver the two forms were equally active. Three different BGH preparations studied in the animal systems were 26–33% as active as LGH. The receptor activity of these BGH preparations was greater than expected from their usBGH content, suggesting that urealabile BGH also binds to the LGH receptor.

When %¹²⁵I-usBGH was employed as radioligand, we found that in the presence of 2, 000 ng/ml of LGH, which caused maximal displacement of 125I-usBGH, the addition of 2 ng/ml of usBGH produced additional displacement. This suggested the presence of a receptor specific for usBGH. However, the phenomenon proved to be due to a contaminant in the usBGH preparations which decreased binding of ¹²⁵IusBGH. BGH containing a substantial fraction of usBGH, and "freeze-stable" BGH which is probably identical with usBGH, both failed to displace m I - usBGH in the presence of 2, 000 ng/ml LGH. These observations rule against the existence of a specific receptor for usBGH.

Supported in part by NIH Grant T01-AM05652 and a grant fom The Elsa V. Pardee Foundation.

Dr. Soman was the recipient of a National Institutes of Health Clinical Traineeship (T01-AM05652).

Received June 24, 1976.