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Journal of Clinical Endocrinology & Metabolism Vol. 44, No. 1 56-61
doi:10.1210/jcem-44-1-56
Copyright © 1977 by the Endocrine Society.
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LH and FSH Levels in Urine and Serum of Prepubertal and Pubertal Children Receiving a 3 Hour Infusion of LH-RH

EDWARD O. REITER, ALLEN W. ROOT and GREGORY E. DUCKETT

Ed Wright Pediatric Endocrinology Research Laboratory, All Children's Hospital St. Petersburg, Florida
The University of South Florida College of Medicine, Department of Pediatrics Tampa, Florida 33612

LH-RH was administered to 17 normal prepubertal and 14 pubertal children as well as to 6 GH deficient prepubertal patients. Urinary immunoreactive LH and FSH were measured in acetone extracts of 3 h collections prior to, during, and immediately after a 3 h infusion of 100 βg LH-RH. Peak LH excretion in response to LH-RH was higher in pubertal than prepubertal children. Girls excreted larger quantities of FSH after LH-RH than did boys. Serum LH and FSH increments evoked by LH-RH correlated significantly (P < 0.01) with peak urinary gonadotropin excretion. These data suggest that measurement of urinary immunoreactive LH and FSH prior to and after LH-RH administration is clinically useful in evaluation of the reproductive endocrine system of young children and of individuals with low basal levels of gonadotropin.

Supported by National Foundation-March of Dimes Grant Nos. 1-323, C-199, and National Foundation-Basil O'Connor Starter Grant No. 5–71.

Received May 17, 1976.




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Copyright © 1977 by The Endocrine Society