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Immunoreactive Luteinizing Hormone, Follicle Stimulating Hormone and Their Subunits in Human Urine Following Gel Filtration

The Vincent Memorial Hospital (Gynecological Service of the Massachusetts General Hospital Boston, Massachusetts 02114

Urine collected from postmenopausal women, normal men, women and children was prepared by vacuum dialysis and kaolin extraction prior to chromatography on Sephadex G-100. Specific radioimmunoassays for LH, subunit, LHβ, FSH and FSHβ were utilized to define the elution patterns of the urinary filtrates, extracts, 2nd IRP-HMG and postmenopausal serum. The following findings were obtained: 1) FSH eluted as a distinct immuno-reactive peak whereas LH eluted more diffusely, 2) free a subunit was present in all urinary preparations, the 2nd IRP-HMG and postmenopausal serum, 3) free FSHβ but not LHβ was detectable in postmenopausal urine, and 4) an LHβ immunoreactive fragment with an approximate molecular weight of 5000 daltons was present in all urinary preparations and the 2nd IRP-HMG but not in postmenopausal serum.

Supported by Grant HD07196-03 and General Research Support Grant RR-5486-12 from National Institutes of Health, and the Vincent Research Fund.

Received February 11, 1976.