This Article Full Text (PDF) Submit a related Letter to the Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by JACOBI, G. H. Articles by WILSON, J. D. Search for Related Content PubMed PubMed Citation Articles by JACOBI, G. H. Articles by WILSON, J. D.

Formation of 5-Androstane-3,17β-diol by Normal and Hypertrophic Human Prostate

Department of Internal Medicine and the Eugene McDermott Center for Growth and Development, The University of Texas Southwestern Medical School Dallas, Texas 75235

Requests for reprints should be addressed to Dr.Wilson.

The 3-keto reduction of [l,2-³H]dihydrotestosterone to 3-and 3β-androstanediols was assessed in homogenates of 40 prostates obtained either at surgery or at medicolegal autopsy from men who had died suddenly. Formation of both androstanediols was demonstrable in cytosol and in microsomes, and both NADH and NADPH were effective cofactors for the two reactions. Formation of the two steroids was not influenced by storage of the gland for up to 8 h prior to processing. When NADPH was cofactor, the formation of 3and 3β-androstanediol was significantly higher in microsomes and cytosol from hypertrophic than from normal glands.

This work has been aided by grant AMO3892 from the U.S. Public Health Service. Dr. Jacobi was the recipient of postdoctoral fellowship Ja277/1 from the Deutsche Forschungsgemeinschaft.

Received June 1, 1976.

This article has been cited by other articles:

				S. Steckelbroeck, Y. Jin, S. Gopishetty, B. Oyesanmi, and T. M. Penning Human Cytosolic 3{alpha}-Hydroxysteroid Dehydrogenases of the Aldo-keto Reductase Superfamily Display Significant 3{beta}-Hydroxysteroid Dehydrogenase Activity: IMPLICATIONS FOR STEROID HORMONE METABOLISM AND ACTION J. Biol. Chem., March 12, 2004; 279(11): 10784 - 10795. [Abstract] [Full Text] [PDF]

				C. S. Cook, L. M. Berry, R. H. Bible, J. D. Hribar, E. Hajdu, and N. W. Liu PHARMACOKINETICS AND METABOLISM OF [14C]EPLERENONE AFTER ORAL ADMINISTRATION TO HUMANS Drug Metab. Dispos., November 1, 2003; 31(11): 1448 - 1455. [Abstract] [Full Text] [PDF]