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A Bioassay for NSILA-S in Individual Serum Samples and Its Relationship to Somatotropin

Medical Research Centre, Prince Henry's Hospital, Melbourne 3004, Australia

A practical bioassay for the acidethanol soluble non-suppressible insulin-like activity (NSILA-S) of individual serum samples has been developed utilizing the incorporation of ¹⁴C-glucose into the lipid faction of isolated adipocytes. NSILA-S activity was correlated with somatotropin status. Thus, the mean potencies (±SD) relative to an extract of pooled normal human serum were: normal samples 1.11 ±0.14, acromegalic 2.91 ± 0.72, and somatotropin deficient 0.13 ± 0.06. This variation in NSILAS was not due to variability in extraction recoveries. The within assay precision was 9% (coefficient of variation) and the between assay 23%. This method allows the simultaneous extraction and processing of relatively large numbers of samples, and compares favorably with other more complex methods. Because of the evidence that NSILA-S may be related to the somatomedins, the present method should provide a simpler and more reliable alternative to the cartilage bioassays used to measure somatomedin activity.

Supported by a Grant from the National Health and Medical Research Council of Australia.

¹ Postgraduate Scholar, National Health and Medical Research Council of Australia.

Received March 15, 1976.

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