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Journal of Clinical Endocrinology & Metabolism, Vol 43, 1164-1169, Copyright © 1976 by Endocrine Society

ARTICLES

A bioassay for NSILA-S in individual serum samples and its relationship to somatotropin

RC Franklin, GC Rennie, HG Burger and DP Cameron

A practical bioassay for the acid-ethanol soluble non-suppressible insulin-like activity (NSILA-S) of individual serum samples has been developed utilizing the incorporation of 14C-glucose into the lipid faction of isolated adipocytes. NSILA-S activity was correlated with somatotropin status. Thus, the mean potencies (+/-SD) relative to an extract of pooled normal human serum were: normal samples 1.11 +/- 0.14, acromegalic 2.91 +/- 0.72, and somatotropin deficient 0.13 +/- 0.06. This variation in NSILA-S was not due to variability in extraction recoveries. The within assay precision was 9% (coefficient of variation) and the between assay 23%. This method allows the simultaneous extraction and processing of relatively large numbers of samples, and compares favorably with other more complex methods. Because of the evidence that NSILA-S may be related to the somatomedins, the present method should provide a simpler and more reliable alternative to the cartilage bioassays used to measure somatomedin activity.


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J. W. Cardinal, D. J. Allan, and D. P. Cameron
Differential Metabolite Accumulation May Be the Cause of Strain Differences in Sensitivity to Streptozotocin-Induced {beta} Cell Death in Inbred Mice
Endocrinology, June 1, 1998; 139(6): 2885 - 2891.
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