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Journal of Clinical Endocrinology & Metabolism, Vol 43, 842-846, Copyright © 1976 by Endocrine Society

ARTICLES

Stimulation of RNA and protein synthesis in isolated chondrocytes by human serum

JT Garland and F Buchanan

The stimulation of isolated chicken embryo chondrocytes was studied by measuring the incorporation of [3H]uridine and [3H]leucine into cold trichloroacetic acid precipitable material after exposure of the chondrocytes to serum. The doseresponse relationships for the incorporation of uridine and leucine were similar to that of thymidine previously demonstrated. Exposure of the cells to serum-containing buffer for 15 min sufficed both for the stimulation of incorporation into the cells and for the depletion of 28% of the stimulating activity from the medium. Stimulation persisted for at least 17 h after removal of the serum. Studies where actinomycin D was added to inhibit RNA synthesis suggested that prior RNA synthesis was required for most of the stimulation of protein synthesis by serum factors.




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Copyright © 1976 by The Endocrine Society