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Journal of Clinical Endocrinology & Metabolism, Vol 43, 712-715, Copyright © 1976 by Endocrine Society

ARTICLES

Competition by 24,25-dihydroxycholecalciferol in the competitive protein binding radioassay of 25-hydroxycalciferol

JG Haddad Jr, C Min, J Walgate and T Hahn

24,25-dihydroxycholecalciferol (24,25-(OH)2D3) is equipotent to 25- hydroxycholecalciferol in the displacement of 3H25-OHD3 from rat serum binding sites, and is extracted by the ethanol procedure recommended in non-chromatographic competitive protein binding radioassays for 25-OHD. Serum 24,25-(OH)2D content was measured following 24,25-(OH)2D3 isolation from lipid extracts by liquid-gel partition chromatography. Since normal serum 24,25-(OH)2D concentration is appreciable, non- chromatographic 25-OHD assays most probably overestimate serum 25-OHD levels as a result of their recognition of 24,25-(OH)2D3.




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Copyright © 1976 by The Endocrine Society