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Improvement in the Quality and Quantity of Growth Hormone Isolated from Acetone-Preserved Human Pituitaries for Clinical Usage

The continuing need for, and inadequate availability of, growth hormone (GH) for clinical usage has directed our attention to the development of an improved isolation procedure. Our aims have been to increase both the yield and also the chemical purity of GH derived from acetone-preserved human pituitaries. This letter describes the achievement of both of these aims.

The characterization of our GH preparation was accomplished by a) gel filtration on long, analytical Sephadex columns (which revealed itto be primarily monomeric); b) radioimmunoassays for glycoprotein hormone contaminants (which revealed it to be free of such contaminants); and c) bioassay, to ascertain the yield of biologically active GH.