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Journal of Clinical Endocrinology & Metabolism Vol. 42, No. 5 894-900
doi:10.1210/jcem-42-5-894
Copyright © 1976 by the Endocrine Society.
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Urine Aldosterone Radioimmunoassay: Validation of a Method Without Chromatography

RONALD D. BROWN, ANNA SWANDER and JOHN K. McKENZIE

Departments of Medicine, Mayo Clinic Rochester, Minnesota Baylor College of Medicine Houston, Texas The University of Manitoba, General Centre, Health Sciences Centre Winnipeg, Manitoba, Canada

Reprints: R. D. Brown, M.D., Mayo Clinic, Rochester, Minnesota 55901.

A simplified radioimmunoassay of urinary aldosterone is reported. Acid-hydrolyzed urine was extracted with dichloromethane and the extract assayed without further purification. Urinary aldosterone values in patients with Cushing's syndrome, low and normal-renin essential hypertension, congential adrenal hyperplasia, and primary aldosteronism determined by this method agreed closely (r = 0.95, P < 0.01) with values obtained using a standardized chromatographic method. This simplified assay represents a significant advance in our capabilities for evaluating patients for abnormalities in aldosterone physiology.

Supported in part by Baylor Clinical Research Center RR 00134, by funds from the Mayo Foundation (Doctor Brown), and by the Canadian Medical Research Council Grant No. MA 4044 (Doctor McKenzie).

Received July 25, 1975.




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E. ARTEAGA, E. G. BIGLIERI, C. E. KATER, J. M. LOPEZ, and M. SCHAMBELAN
Aldosterone-Producing Adrenocortical Carcinoma: Preoperative Recognition and Course in Three Cases
Ann Intern Med, September 1, 1984; 101(3): 316 - 321.
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Copyright © 1976 by The Endocrine Society