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Journal of Clinical Endocrinology & Metabolism, Vol 42, 561-566, Copyright © 1976 by Endocrine Society

ARTICLES

Measurements of free thyroxine: comparison of per cent of free thyroxine in diluted and undiluted sera

H Uchimura, S Nagataki, T Tabuchi, M Mizuno and SH Ingbar

The effect of dilution on values of the per cent of free T4 in serum (%FT4) measured by equilibrium dialysis, was determined by comparing the values for %FT4 in diluted and undiluted specimens. The specimens studied were sera obtained from 10 normal subjects, 10 patients with hyperthyroidism, 5 with hypothyroidism, and 9 women with normal pregnancy. The method of analysis employed was that described by Sterling and Brenner, except that the 125I-thyroxine used was predialyzed to remove non-thyroxine contaminants and that "corrected" values for %FT4 were calculated by dividing measured values by the numerical extent to which the serum had been diluted.


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