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Journal of Clinical Endocrinology & Metabolism, Vol 42, 189-192, Copyright © 1976 by Endocrine Society

ARTICLES

A new extractant for serum thyroxine by enzymatic digestion of thyroxine binding proteins

KH Chau and LM Cummins

The use of a pepsin solution to digest serum proteins results in quantitative release of thyroxine from carrier proteins. This extraction method greatly simplifies the original Murphy and Pattee (1) competitive protein-binding assay for serum thyroxine. The simplicity and efficiency of this extractant suggest that it may have application in radioimmunoassay and competitive protein-binding assay for nonpeptide ligands in serum.




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Copyright © 1976 by The Endocrine Society