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Journal of Clinical Endocrinology & Metabolism Vol. 41, No. 1 131-134
doi:10.1210/jcem-41-1-131
Copyright © 1975 by the Endocrine Society.
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Thyrotropin-Releasing Hormone (TRH): Measurements in Human Spinal Fluid1

GEORGE E. SHAMBAUGH, III, JOHN F. WILBER, ELADIO MONTOYA2, HENRY RUDER and E. RICHARD BLONSKY

Department of Nutrition, Endocrinology and Metabolism, Northwestern University Medical School and Departments of Medicine and Neurology Northwestern Memorial Hospital, and Veterans Administration Research Hospital Chicago, Illinois

Reprints: Dr. George E. Shambaugh III, Veterans Administration, Research Hospital, 333 E. Huron Street, Chicago, Illinois 60611.

Levels of thyrotropin-releasing hormone (TRH) were quantitated in human lumbar spinal fluid (CSF) utilizing a sensitive and specific TRH radioimmunoassay. Endogenous TRH was sufficiently stable in CSF to permit 85% recovery of intact TRH after 48 h storage at 4 C. TRH levels in AM or PM samples obtained from 15 women and 12 men were easily detected in all CSF specimens. No significant difference between the TRH concentration in CSF of men and women was observed (44.2 ± 6.8 and 38.1 ± 6.5 pg/ml (mean ± SE) respectively). TRH concentrations were 40.2 ± 6.9 pg/ml (mean ± SEM) in AM and 41.4 ± 8.0 pg/ml in PM samples. By contrast, CSF cortisol levels obtained concurrently were twofold higher in AM than PM (0.68 ± 0.08 vs 0.38 ± 0.02 µg/100 ml (mean ± SEM) respectively, P < 0.001). These data are consistent with the possibility that a portion of the TRH in CSF can be derived from the central nervous system (CNS) and unrelated to the hypophysiotropic control of thyrotropin (TSH) synthesis and secretion.

1 Supported in part by Research Grants AM-10699, and AM-14997, and Training Grant AM-05071 from the National Institute of Arthritis Metabolic and Digestive Diseases, U.S.P.H.S., Bethesda, Maryland, Grant FR-05470 from the National Institutes of Health, Bethesda, Maryland; a Schweppe Foundation Grant and the Chicago Wesley Memorial Hospital Cancer Research Fund, Chicago, Illinois.

2 Dr. Montoya is a recipient of a Research Fellowship from the Commission for Cultural Exchange between Spain and the USA.

Received January 14, 1975.






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Copyright © 1975 by The Endocrine Society