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Journal of Clinical Endocrinology & Metabolism Vol. 40, No. 5 863-867
doi:10.1210/jcem-40-5-863
Copyright © 1975 by the Endocrine Society.
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A Nonchromatographic Radioimmunoassay for 17{alpha}-Hydroxyprogesterone

MIRIAM HAMI, ARIEL ROSLER and DAVID RABINOWITZ

Department of Chemical Endocrinology, Hadassah University Hospital Jerusalem, Israel

A radioimmunoassay is described for the measurement of 17{alpha}-hydroxyprogesterone in human plasma. We have employed the principle of "immunologic purification." 17-OHP and related steroids are bound to an excess of antiserum. Steroids with a low affinity for the antibody are extracted by ether. The 17-OHP is subsequently freed from antibody by acid hydrolysis and this extract is assayed by radioimmunoassay. (J Clin Endocrinol Metab 40: 863, 1975)

Received October 17, 1974.






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Copyright © 1975 by The Endocrine Society