help button home button Endocrine Society JCEM JCEM Call for Nominations for EIC
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Submit a related Letter to the Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stern, M. D.
Right arrow Articles by Givner, M. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stern, M. D.
Right arrow Articles by Givner, M. L.

Journal of Clinical Endocrinology & Metabolism, Vol 40, 728-731, Copyright © 1975 by Endocrine Society

ARTICLES

Measurement of serum clogestone acetate (AY-11,440) by a radioreceptor assay: a practical approach to the quantitative determination of synthetic progestins

MD Stern and ML Givner

A novel method is described for the measurement of nanogram quantities of clogestone acetate (3beta, 17alpha-dihydroxy-6-chloropregna-4,6-dien- 20-one 3,17-diacetate) in serum:clogestone acetate (CgAc) is converted to chlormadinone acetate in the presence of wheat germ lipase and hydroxysteroid dehydrogenase. The ketonic steroid formed is then incubated with rat uterine cytosol and 3-H-progesterone. The concentration of 3CgAc is estimated from a standard curve derived by incubating cytosol with 3-H-progesterone and varying amounts of chlormadinone acetate. The statistically validated method has been used for the estimation of serum CgAc in humans and dogs given an oral dose of the steroid. The radioreceptor assay (RRA), has practical advantages over related techniques such as radioimmunoassay (RIA) especially with respect to the developmental work. This is the first time that a quantitative assay of a progestin by the tissue receptor approach has been described.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1975 by The Endocrine Society