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BINDING OF CORTICOSTEROIDS BY PLASMA PROTEINS. VI. THE BINDING OF CORTISOL AND ALDOSTERONE BY CORTICOSTEROID-BINDING GLOBULIN AND BY THE ESTROGEN-INDUCED BINDING SYSTEM OF PLASMA^*

WILLIAM H. DAUGHADAY, M.D., JOHN HOLLOSZY, M.D. and KOZAK MARIZ IDA, B.A.

Metabolism Division, Department of Medicine, Washington University School of Medicine St. Louis, Missouri

The binding of cortisol-4-C¹⁴ and aldosterone-H³ by serum from pregnant or estrogen-treated subjects was directly compared to the binding by serum from control subjects in a system of double equilibrium dialysis. Increased binding of cortisol-4-C¹⁴ could be demonstrated by "estrogen" plasma under conditions of minimal cortisol loading only at 37°C. and not at 4°C. This difference of binding behavior is evidence that the binding sites induced by estrogen are qualitatively different from those on normal corticosteroid-binding globulin. The total concentration of cortisol-binding protein induced by estrogens may be two or three times greater than that of corticosteroid-binding globulin. Under physiologic conditions of temperature, aldosterone-H³ is bound primarily by albumin, but at 4° C. aldosterone-H³ is bound about equally by albumin and corticosteroid-binding globulin. At 4° C. the estrogen-induced binding system does not have appreciable affinity for aldosterone-H³.

^* Presented in part at the Conference on the Biological Activity of Steroids in Relation to Cancer, sponsored by Endocrinology Section, Cancer Chemotherapy, National Service Center, National Institutes of Health, Vergennes, Vermont, September, 1959 (1).

Assistant Resident in Medicine, St. Louis Jewish Hospital.

Received June 9, 1960.